nCounter Core Facility

Overview of Services

The nCounter Core Facility has been established to provide access to one of the current state of the art target gene profiling technologies for the Heidelberg University research community. It is supported by the Excellence Cluster Cell Networks and the DFG (German Research Society).

 

Our service includes samples quality control (QC), hybridization, samples processing, nCounter analysis, data QC and temporary storage and data delivery. 

 

Data analysis by the nCounter Core Facility includes QC. For detailed data analysis nanoString provides the nSolverTM Analysis Software. This programme enables quick and easy QC, normalisation, and analysis of data without the need to purchase additional software packages. Furthermore, data visualisation including box plots, heat maps, scatter plots, violin plots and histogrammes is possible.

 

 

The nCounter system from Nanostring Technologies is a complete, fully automated system for the next generation of digital gene expression analysis.

 

The nCounter system is an instrument designed for multiplexed measurement of gene expression using fluorescently labeled reporter probes, so called ‘codesets’. The codeset probes are ca 100 bases in length. Therefore, the system is very resistant to lower RNA quality and is perfectly suited for critical samples such as FFPE (formalin-fixed, paraffin-embedded) samples.

Applying a unique coding technology enables direct counting of individual RNA molecules across all levels of biological expression, with sensitivity and specificity comparable to Real Time PCR (RT PCR). The main advantage is that no enzymatic reactions are involved, in particular no reverse transcription is necessary.

 

Applications:

  • mRNA Profiling
    Custom set of genes or off-the-shelf preassembled panels (details on NanoString website).
  • miRNA Profiling
    Comprehensive collection of more than 700 human and human-associated viral miRNAs as well as murine miRNAs derived from miRBase (details on NanoString website).

 Scientists interested in using the facility are welcome to contact us by mail at: n.counter@med.uni-heidelberg.de


Leadership

PD Dr. Beate Niesler, Head of Facility

Tel:  0049 (6221) 56-35274
Email:  beate.niesler@med.uni-heidelberg.de

 

Dipl.-Ing. Ralph Röth, Technical staff
Heike Kuzan, Technical staff

Tel: +49 6221 56-34472
Email: n.counter@med.uni-heidelberg.de

 

Location and Hours of Operation

Hours - Staffed

Hours - Sample Submission

Location

 

MON - FRI  9:00 - 17:00

 

 


Upon prior consultation

 

  Department of Human Molecular Genetics
  Heidelberg University Hospitals
  Im Neuenheimer Feld 366
  69120 Heidelberg
  Fon +49 6221 56-35274; Fax +49 6221 56-5155
  E-mail: beate.niesler@med.uni-heidelberg.de

Links and Resources

For more information, please visit:

 

The nCounter Core Facility Official Website

The NanoString Website

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Contacts

Name Role Phone Email Location
PD Dr.rer.nat. Beate Niesler
Scientist
 
0049 (6221) 56-35274
 
beate.niesler@med.uni-heidelberg.de
 
Room 507
 
Dipl. Ing. Ralph Röth
Technican
 
0049 (6221) 56-34472
 
n.counter@med.uni-heidelberg.de
 
Room 523
 
Heike Kuzan
Technican
 
0049 (6221) 56-34472
 
n.counter@med.uni-heidelberg.de
 
Room 523
 

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