nCounter Core Facility

Overview of Services

The nCounter Core Facility was established in 2011 and is part of the CellNetworks Core Technology Platform at Heidelberg University and the MULTI-SPACE platform of the Health & Life Science Alliance.
The facility offers state-of-the-art methods for mRNA and miRNA expression profiling (nCounter SPRINT Profiler), as well as Digital Spatial Profiling at the transcript level (GeoMx Digital Spatial Profiler) and protein level (GeoMx Digital Spatial Profiler, Lunaphore COMET).
In addition, the facility provides various analytical methods for quality control of RNA and DNA samples.
In the DFG’s research infrastructure portal "RIsources", the nCounter Core Facility is listed under RI_00563 (nCounter SPRINT Profiler, GeoMX Digital Spatial Profiler) and RI_00613 (Lunaphore COMET).

 

 

 

 

 

Bruker Spatial Biology Platform Service includes:

 

  • nCounter SPRINT Profiler expression analysis
  1. Support of project planning
  2. Quality control (QC) of input material
  3. Performance of nCounter SPRINT Profiler experiment
  4. QC of generated data and data delivery
  5. Temporary data and sample storage
  6. Support in data analysis applying the nSolver software
  7. in case of cooperation*: data analysis and provision of results

 

 

  • GeoMx Digital Spatial Profiler analysis
  1. Support of project planning together with Bruker Spatial Biology
  2. Technical assistance
  3. Performance of nCounter SPRINT Profiler protein or RNA readout
  4. Data delivery
  5. Temporary data and sample storage

 

  • Quality Control Only (RNA/DNA)
  1. Support of project planning
  2. Performance of TapeStation or/and Qubit analysis
  3. Data delivery
  4. Temporary data and sample storage

 

 

Lunaphore Platform Service includes:

 

!!!! Under Construction !!!!

 

Leadership

Dr. Stefanie Mellein, Head of Facility

Tel:  +49 6221 56-35274
Email:  stefanie.mellein@med.uni-heidelberg.de

 

Dipl.-Ing. Ralph Röth, Bruker Spatial Biology Platform, Technical staff

Heike Kuzan, Bruker Spatial Biology PlatformTechnical staff

Tel: +49 6221 56-34472
Email: n.counter@med.uni-heidelberg.de

 

Dr. Esther Kimmle, Lunaphore Platform

Tel: +49 6221 56-311563
Email: esther.kimmle@uni-heidelberg.de      

 

Location and Hours of Operation

 

Hours - Staffed Hours - Sample Submission Location/Bruker Spatial Biology Platform
MON - FRI  9:00 - 17:00 Upon prior consultation Department of Human Genetics
Heidelberg University Hospitals
Im Neuenheimer Feld 366, 5th Floor, Room 523
69120 Heidelberg
Fon +49 6221 56-34472; Fax +49 6221 56-8884
E-mail: n.counter@med.uni-heidelberg.de      
     
Hours - Staffed Hours - Sample Submission Location/Lunaphore Platform
MON - FRI  9:00 - 17:00 Upon prior consultation Department for Computational Biomedicine
Heidelberg University Hospitals
Im Neuenheimer Feld 130.3, 10th Floor
69120 Heidelberg
Fon +49 6221 56-311563
E-mail: esther.kimmle@uni-heidelberg.de      

Links and Resources

For more information, please visit:

 

The nCounter Core Facility Official Website

The Bruker Spatial Biology Website

Cellnetworks Core Technology Platform (CCTP)

Health + Life Science Alliance Heidelberg Mannheim

 

Funding

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Contacts

Name Role Phone Email Location
Dipl. Ing. Ralph Röth
Technican
 
0049 (6221) 56-34472
 
n.counter@med.uni-heidelberg.de
 
Room 523
 
Heike Kuzan
Technican
 
0049 (6221) 56-34472
 
n.counter@med.uni-heidelberg.de
 
Room 523
 
Dr. rer. nat. Stefanie Mellein
Scientist
 
0049 (6221) 56-35274
 
stefanie.mellein@med.uni-heidelberg.de
 
Room 507
 
Dr. Esther Kimmle
Scientist
 
0049 (6221) 56-311563
 
esther.kimmle@uni-heidelberg.de
 

 

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