Metabolomics Core Technology Platform

 

Overview of Core

The Metabolomics Core Technology Platform offers a broad range of qualitative and quantitative metabolite analyses. We are located at the Centre for Organismal Studies Heidelberg (COS Heidelberg) and are member of the HMLS Core Facility program. All researchers of the University of Heidelberg, the Heidelberg and Mannheim University Hospital, DKFZ, MPImF and EMBL are welcome to request help or place orders for metabolite analyses.

Before first sample submission, please schedule an initial meeting to discuss your project in detail.

Services

The MCTP provides analytical services for untargeted and targeted metabolite analyses.

Untargeted analyses can be performed for comprehensive metabolite fingerprinting by LC-MS/MS leading to a spectrum of mainly primary metabolites (e.g. members of Citric Acid Cycle).

The evaluation of accomplished projects since the start of MCTP reveals a significant demand for targeted analyses of customer-defined metabolites.
Therefore the MCTP performs targeted metabolite profiling and single compound analysis. This allows us to routinely analyze and quantify the following classes of compounds:

-      Inorganic ions (anions and cations)
-      Carbohydrates / sugars
-      Citrate cycle compounds (dicarboxylic organic acids, e.g. malate, fumarate, citrate)
-      a-Ketoacids (e.g. glyoxylate, pyruvate, ketoglutarate)
-      Amino acids
-      Polyamines
-      Nucleotides (e.g. ADP, ATP, GTP, CTP, UTP)
-      Thiols (glutathione and cysteine)

A detailed list of all compounds which have been measured and quantified so far is available upon request.

For the targeted analysis of additional metabolites please contact the MCTP.

Our service includes extraction, separation and detection of metabolites and basic bio-informatics of your data. 

Matrices

The so far analyzed sample matrices include metabolite extracts of

-      Prokaryotes
-      Yeast
-      Cell culture (Drosophila and human)
-      Human, animal and plant tissues
-      Human body fluids and cell culture/bacterial supernatants

Measurements of further matrices can be established upon request.

Current Equipment

-    GC-MS system (Shimadzu) for semi-quantitative, comparative studies of primary metabolism

-    HPLC systems (Waters and Dionex) equipped with fluorescence, UV, conductivity and pulsed
     amperometric detectors for targeted analyses

-    UPLC system coupled to a XEVO qTOF MS (Waters) for unbiased comparative studies.

-    UPLC system coupled to PDA-, FLR- and QDA - detectors (Waters) for targeted analyses. 

Leadership Personnel

Prof. Dr. Rüdiger Hell  Scientific Director

Tel.: +49 (6221) 546284    Email: ruediger.hell@cos.uni-heidelberg.de

Location and hours of operation

Hours - Staffed Hours - Sample Submission Location
 

MON - FRI  9:00 - 17:00

 

 

 

MON and THU 9:30 – 11:30 and 13:00 – 16:00

TUE and FRI 9:30 – 11:30

Other times upon prior consultation

Metabolomics Core Technology Platform 

Im Neuenheimer Feld 360 - Room -118

69120 Heidelberg

Links and Resources 

  1. Center for Organismal Studies Heidelberg
  2. General Guideline for Metabolite Analyses
  3. End-User License Agreement

Contacts

Name Role Phone Email Location
Dr. Gernot Poschet
scientist
 
+49 (6221) 54-5536
 
gernot.poschet@cos.uni-heidelberg.de
 
Room -118
 
Dr. Michael Büttner
scientist
 
+49 (6221) 54 5536
 
michael.buettner@cos.uni-heidelberg.de
 

 
Eva-Maria Käshammer-Lorenz
technician
 
+49 (6221) 54-5536
 
eva-maria.kaeshammer@cos.uni-heidelberg.de
 

 
Lorenz Nowack
technician
 
+49 (6221) 54-5536
 
lorenz.nowack@cos.uni-heidelberg.de
 

 

Service list


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Quantification Services (16)
Additional Services (3)

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